摘要
目的探索并建立一种检测树鼩群体遗传多样性的方法。方法利用聚合酶链反应(PCR)扩增技术对30只树鼩个体的11个微卫星位点进行了遗传检测。结果所选的11个微卫星DNA位点中,有9个具有高度多态性,2个微卫星DNA位点多态性较差。结论所研究的树鼩微卫星位点中,有9个符合遗传标记特点,可用于检测树鼩群体的遗传多样性。
Objective In order to explore and set up an accurate and convenient method for genetic assessment of tree shrews.Method DNA polymorphic microsatellites of Tupaia Belangeri chinensis were analyzed using PCR technique in 30 tree shrews.Results Nine polymorphic microsatellites loci,2 non-polymorphic microsatellites loci were screened.Conclusion Nine of eleven DNA polymorphic microsatellites 1oci can be used for genetic assessment of tree threw.
出处
《中国实验动物学报》
CAS
CSCD
2009年第2期143-145,共3页
Acta Laboratorium Animalis Scientia Sinica
基金
云南省科技基础条件平台建设计划项目(2006PT07-1)
关键词
树鼩
微卫星DNA
多态性
Tree threw
Microsatellites DNA
Polymorphism
