白芷香豆素的镇痛机制初探

Mechanism Underlying Analgesic Effect of Coumarin of Angdicae dahuricae

目的探讨白芷香豆素（Coumarin of Angelicae dahuricae，CAD）的镇痛作用机制．方法采用热板法，通过工具药观察CAD的镇痛作用与阿片受体和单胺类神经递质的关系；利用硝酸还原酶法检测CAD对甲醛所致伤害性疼痛模型小鼠血清一氧化氮（NitricOxide，NO）的含量．结果纳洛酮（5mg／kg）部分拮抗CAD（60mg／kg）的镇痛作用；利血平（4mg／kg）可以部分拮抗CAD（60mg／kg）的镇痛作用．CAD（30，60，120mg／kg）连续给药4d，使甲醛所致伤害性疼痛模型小鼠血清NO含量明显下降．结论CAD具有明显的镇痛，其镇痛作用与阿片受体和脑内单胺类神经递质有一定的关系．此外，明显减少NO的合成可能是其发挥镇痛作用的重要机制．

To observe the mechanism underlying analgesic effect of Coumarin of Angdicae dahuricae （CAD）. Method The analgesic effect of CAD in mice was observed by using hot-plate, the relationship between analgesic effect of CAD and opioid receptor as well as monoamine neurotransmitter was observed through several agents;The concentration of nitric oxide（NO） in serum of analgesic mice induced by formaldehyde was measured with nitric reductase method. Results Naloxone （5 mg/kg）and reserpine （4 mg/kg） antagonized in part the analgesic effect of CAD （60 mg/kg） ;After four-day administration of CAD （30.60.120 mg/kg）, the concentration of NO in serum of noeiceptive pain mice reduced Dy formaldehyde significantly decreased. Conclusion CAD has definite analgesic effect. There is a relationship between analgesic effect and opioid receptor as well as monoamine neurotransmitter. It is very important mechanism that CAD reduced production of NO.