应用胶体金层析法快速检测鼠疫耶尔森氏菌F1抗原

Rapid Detection of the Fraction 1(F1) Antigen of Yersinia pestis with Colloidal Gold-immunochromatography

目的研究胶体金免疫层析法用于鼠疫耶尔森菌的快速诊断。方法采用胶体金免疫层析测试条,加入待测鼠疫耶尔森菌液及对照菌液。结果金标免疫层析试纸条在检测中并未出现交叉反应,特异性达到100%,显示其在鼠疫耶尔森菌的检测中特异性较好,结果可靠。同时该试纸条敏感性亦较高,少量细菌或抗原就可得到阳性结果,检测效果好。结论该方法简便、快速、敏感、特异性好,适合于公共突发卫生事件中的鼠疫快速诊断和现场鼠疫监测。

Objective To develop a method for rapid detection of the fraction-1 （F1） antigen of Yersinia pestis. Method The purified F1 antibody was coupled with colloidal gold to be used for the detection of F1 antigen by means of the double antibody sandwich. The sensitivity, specificity and stability of this method of detection were evaluated by the testing with 20 different strains of enteropathogenic bacterias and several concentrations of bacterial suspension and the solution of purified F1 antibody. Result The detection method demonstrated a 100% specificity, and satisfactory sensitivity. Conclusion It is concluded that a simple and rapid colloidal gold-immunochromatography assay for the detection of F1 antigen Y.pestis is established in this study.