施用沼肥对设施土壤固氮菌影响的研究

Effects of applying biogas fertilizer on nitrogen-fixation bacteria in greenhouse soil

以有机质含量相同的猪粪、沼渣为基肥,以N,P,K含量相同的化肥与沼液为追肥,以L402番茄为供试作物,采用二裂式区组设计的方法,研究了沼肥、猪粪、化肥等不同施肥组合对设施土壤固氮菌动态变化的影响。试验结果表明:随着番茄生育期的推进,各施肥处理土壤自生固氮菌的数量逐渐增加,基施沼渣比基施猪粪有利于促进土壤自生固氮菌的生长;从番茄苗期到成熟期,追施化肥土壤自生固氮菌数量多于追施沼液土壤,猪粪与化肥配合施用、沼渣与沼液配合施用分别较猪粪与沼液配合施用、沼渣与化肥配合施用能更好地促进土壤自生固氮菌的繁殖。

The paper studied the effects of the combination of biogas fertilizer,pig manure and chemical fertilizer on nitrogen-fixation bacteria in greenhouse soil. In the experiment,IA02 tomato is chosen as sample ,split-plot design is adopted and pig manure, biogas sludge are utilized as base fertilizers in which the organic matter contents are equivalent,chemical fertilizer and biogas slurry are used as top fertilizers in which the N,P and K contents are equivalent .The results are as followings：along with growth progress, the amount of nitrogen-fixation bacteria in the soil is becoming more and more in each fertilization treatment. Applying biogas sludge as base fertilizer is more fa- vorable to promote nitrogen-fixation bacteria growth than that of applying pig manure. From tomato seedling to ripening period ,the amount of nitrogen-fixation bacteria is much more with chemical fertilizer as top fertilizer than that with biogas slurry as top fertilizer. Applying pig manure and chemical fertilizer,biogas sludge and biogas slurry have better effects in increasing the amount of nitrogen-fixation bacteria than those of applying pig manure and biogas slurry,biogas sludge and chemical fertilizer, respectively.