摘要
目的对比广西壮、汉民族HLA-DRB1*07基因与乙肝疫苗免疫应答的相关性。方法以广西73名壮族、97名汉族健康大学生作为研究对象进行全程乙肝疫苗接种,采用PCR-SSP技术检测受试者外周血细胞DNA的HLA-DRB1*07基因,采用ELISA方法检测其血清乙肝病毒标志物(HBsAg,抗-HBs,HBeAg,抗-HBe,抗-HBc)。结果壮族与汉族之间乙肝疫苗的应答率无显著性差别(84.21%vs 86.60%,P=0.658),壮族与汉族人群之间HLA-DRB1*07基因携带频率的差异也无统计学意义(9.21%vs 6.19%,P=0.454),但壮、汉族中HLA-DRB1*07携带者的疫苗应答率都显著低于非携带者(P=0.00 P/=0.03)。结论民族差异不是广西人群乙肝疫苗应答效果的影响因素;广西壮、汉族人中携带HLA-DRB1*07基因都可能是导致机体对乙肝疫苗无/弱应答的因素。
Objective To investigate the relationship between HLA-DRB1 * 07 gene and the feature of immune response to hepatitis B vaccine among Zhuang and Han nationality from Guangxi. Methods 76 healthy college students of Zhuang and 97 of Hart nationality from Gnangxi autonomous region were reeeived standard courses of vaeeination with hepatitis B vaccine. HBV makers (HBsAg, anti-HBs, HBeAg, anti-HBe,anti-HBe) of the subjects were tested with ELISA. HLA-DRB1 * 07 was detected with PCR-SSP. Result No significant difference was showed between Zhuang and Han nationality in the immune-responders (84.21% vs 86.60% ,P =0. 658), and there was no significant differenee between the frequency of HLA-DRB1 * 07 in Zhuang and Han nationality (9.21% vs 6.19% ,P=0.454. Both Zhuang and Han nationality of the frequency of immune-responders in the carriers of HLA-DRB1 * 07 gene was significantly lower than that of non-HLA-DRB1 * 07 eaniers( P =0. 000/P = 0.03 ). Conclusions HLA-DRB1 * 07 gene is asseeiated to the non- or hepo-response to hepatitis B vacoine in Zhuang and Han nationality from Guangxi province. The difference of the nationality is not the factor which influences the response to hepatitis B vaccine.
出处
《内科》
2009年第2期181-184,共4页
Internal Medicine
基金
广西壮族自治区卫生厅自筹课题(Z2005062)
广西自然科学基金课题(桂科自0728159)