毒死蜱和甲氨基阿维菌素苯甲酸盐的联合增效作用机理研究

Study on the Synergistic Mechanism of Mixture of Chlorpyrifos and Emamectin Benzoate

以水稻褐飞虱为试虫,从对靶标酶和水解酶抑制作用两个方面,研究了毒死蜱和甲氨基阿维菌素苯甲酸盐联合增效作用机理。结果表明,以毒死蜱为毒力标准,甲氨基阿维菌素苯甲酸盐对乙酰胆碱酯酶、羧酸酯酶和谷胱甘肽S一转移酶的相对毒力指数分别为:0.76、1.52和0.75,与毒死蜱联合抑制作用的相对毒力指数分别为10.41、11.68和7.28,明显高于独立作用。药代动力学研究表明,毒死蜱和甲氨基阿维菌素苯甲酸盐对乙酰胆碱酯酶活性的抑制方式为竞争性抑制。

The synergistic mechanism of a mixture of chlorpyrifos and emamectin benzoate on the larvae of brown rice planthopper（Nilapavata lugens） was studied by the analysis of interaction between chlorpyrifos and emamectin benzoate on target and Detoxicating enzyme. The result showed that the joint action of chlorpyrifos and emamectin benzoate to acetyl cholinestcrase （ACHE）, carboxyl esterase （CarE） and Glutathione - S-ransferase were stronger than independence. Taking chlorpyrifos as standard, the relative toxicity of emamectin benzoate were 0.76,1.52 and 0.75 to ACHE, CarE,and Glutathione - S-ransferase respectively. However, the joint relativetoxicity of emamectin benzoate and chlorpyrifos were 10.41, 11.68 and 7.28. Graphic analysis on the toxicokinetic mode of action on the acetylcholinesterase of joint treatment indicated that the mixture of chlorpyrifos and emamectin benzoate inhibited the enzyme competitively .