高效液相色谱法测定胃欣舒胶囊中大黄酸、大黄素和大黄酚的含量

Determination of rhein,emodin and chrysophanol in Weixinshu capsules by HPLC

目的建立胃欣舒胶囊中大黄酸、大黄素、大黄酚的高效液相色谱法含量测定方法。方法色谱柱:Kro-masil C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液(82∶18);流速:1.0 mL.min-1;检测波长:254 nm。结果大黄酸、大黄素、大黄酚总含量为1%以上,平均回收率分别为97.6%、99.1%、97.7%,RSD分别为2.13%、2.01%、1.73%。结论该方法简便、灵敏、准确,可作为胃欣舒胶囊的质量控制方法。

Objective To establish an HPLC method for the determination of contents of anthraquinones in Weixinshu capsules. Methods Kromasil ODS column （250 mm×4.6 mm, 5μm） was used. The mobile phase contained a mixture of methanol-0.1% phosphoric acid solution （82 ： 18）, flow rate 1. 0 mL·min^-1 , and the wavelength was 254nm. Results The total content of rhein, emodin and chrysophanol was above 1 %. The average recovery rate was 97.6%, 99.1%, 97.7% and RSD was 2.13%, 2.01%, 1.73%. Conclusion The method is simple, accurate, sensitive and can be used to control the quality of Weixinshu capsule.