抗菌肽Thanatin突变体(S-thanatin)对临床耐药革兰阴性菌的体外活性及与7种抗生素的协同作用

Activity of S-thanatin Thanatin Analog against Multidrug-Resistant Gram-Negative Organisms In Vitro and The synergy with 7 kinds of Antibiotics

该研究旨在评价抗菌肽S-thanatin对革兰阴性临床耐药菌大肠埃希菌和肺炎克雷伯菌的体外抗菌活性以及和7种抗生素的协同作用。采用肉汤微量稀释法考察S-thanatin对革兰阴性临床耐药菌抗菌活性以及杀菌作用时间,并用方格滴定法进行协同作用测试。结果所有菌株在S-thanatin浓度为2～16mg/L时都得到抑制,在1h时,绝大部分细菌被杀死。S-thanatin与头孢吡圬联合使用抗肺炎克雷伯菌时有协同作用。研究表明S-thanatin对革兰阴性临床耐药菌有良好的体外抗菌活性,是潜在的抗多药耐药菌药物。

This study was performed to evaluate the in vitro activity of the analog of thanatin, s-thanatin, alone or combined with seven antibiotics against multidrug-resistant gram-negative clinical isolates. The MICs and MBCs were determined by a broth micro dilution method. The antibiotic combinations were tested by a checkerboard titration method. All isolates were inhibited at concentrations of 2 to 16 mg/L,its activity against the gram-negative organisms was complete after 1 h at a concentration of 2 MIC. Synergy was demonstrated when S-thanatin was combined with Cefepime against Klebsislla pneumoniae. S-thanatin showed broad antimicrobial activity against multidrug-resistant gram-negative bacteria, and it is one potential peptide against antibiotic-resistant pathogens.