Cu^(2+)、Zn^(2+)对罗氏沼虾鳃中SOD同工酶的影响

Effects of Cu^(2+),Zn^(2+) on SOD isoenzyme in Macrobrachium rosenbergii's gill

采用生态学单因子梯度试验方法,研究了水环境中添加0.01mg/L,0.08mg/L,0.64mg/L,5.12mg/L的Cu2+,Zn2+对罗氏沼虾鳃中超氧化物歧化酶(SOD)同工酶活力和酶谱的影响.结果显示:随着Cu2+,Zn2+浓度的增加鳃中SOD酶活力均呈先增加后减少的变化趋势,分别在0.64 mg/L Cu2+和0.08 mg/L Zn2+时SOD酶活力达到最大(73.57±0.12 U/mg和53.63±0.17 U/mg),且均与对照组有显著差异(P<0.05);低浓度Cu2+处理组SOD同工酶酶谱出现SOD酶带变宽、颜色加深现象,并在0.08 mg/L和0.64 mg/L浓度组中新增一条酶带(SOD2),而高浓度组(5.12mg/L)明显抑制SOD酶带活性,新增的条带也缺失;同样低浓度的Zn2+使得SOD酶谱的条带变宽,颜色加深,但没有出现新增酶带,而高浓度(5.12 mg/L)Zn2+使得条带变窄,颜色变淡.结果表明:这两种金属离子对罗氏沼虾鳃中SOD同工酶活力及酶谱的影响与浓度有关,Cu2+对SOD的影响明显大于Zn2+,SOD同工酶活力和酶谱的变化可以灵敏地反映Cu2+的胁迫程度和毒性.

Effects of Cu^2＋ and Zn^2＋ in different concentrations （0.01 mg/L, 0.01 mg/L ,0.64 mg/L,5.12 mg/L） on superoxide dismutase（ SOD）＇ s activity and isoenzyme pattern in Macrobrachium rosenbergii＇ s gill were studied by single gradient factor experiments. The result showed that along with the increasing concentration of Cu^2＋ and Zn^2＋ ,SOD＇s activity in gill both ascended at first and then descended , respectively, at 0. 64 mg/L Cu^2＋ and 0. 08 mg/L Zn^2＋ ,got the climax （73.57 ±0. 12 U/rag and 53.63 ±0. 17 U/mg） which were different from the control obviously （P 〈0.05）. SOD isoeryme bands both got wider and darker with the increase of Cu^2＋ in low concentration and one more band （SOD2） was found in 0.08 mg/L and 0. 64mg/L group. While in the high concentration group （5. 12 mg/L） the SOD activity was inhibited obviously and the new band trussed. Zn^2＋ in low concentration enhanced SOD bands and inhibited them when in high concentration group. No new band was found in Zn^2＋ groups. The results indicated that SOD＇ s activity and isenzyme pattern related with the two heavy metal ion＇ s concentration above and Cu^2＋＇ s influence was greater than Zn^2＋ ＇ s obviously. Perhaps the changes of SOD＇s activity and isoenzyme pattern could reflect Cu^2＋ ＇s stress and toxicity.