氧化苦参碱抑制子宫内膜癌细胞株Ishikawa的实验研究

The inhibitory effect of oxymatrine on endometrial cancer cell line Ishikawa

目的:观察不同浓度氧化苦参碱(oxymatrine,Oxy)对人子宫内膜癌细胞株Ishikawa增殖抑制及诱导凋亡作用。方法:培养人子宫内膜癌细胞株Ishikawa,通过细胞形态学观察、CCK-8比色法检测细胞增殖,流式细胞仪检测细胞凋亡及细胞周期人工重组基底膜侵袭小室(transwell)观察细胞的侵袭力。结果:Oxy明显抑制Ishikawa增殖,并呈时效和量效关系,24h、48h、72h的IC50分别为8.07,4.62,3.22mg/ml;细胞周期发生明显改变。随着药物浓度增高,G1期细胞比例增加,S期细胞减少;流式细胞仪可检测到细胞凋亡现象,当Oxy浓度为10mg/ml时,细胞凋亡率65.4%;荧光显微镜可见细胞凋亡形态学变化;Ishikawa细胞侵袭基底膜的能力明显被抑制。结论:Oxy可抑制Ishikawa增殖,诱导Ishikawa细胞凋亡,有可能成为治疗子宫内膜癌的有效药物。

Objective：To investigate the inhibition effect of oxymatrine on the proliferation and apoptosis of endometrial cancer cell line Ishikawa. Methods：Ishikawa cells were treated by oxymatrine at different concentrations. Cell growth and proliferation were observed by CCK-8 assay. Morphological changes of cells were observed. Apoptosis and cell-DNA distribution was analysized by flow cytometry. The effect of Oxymatrine on invasion of cells was evaluated using Transwell chambers attached with polycarbonate filters reconstituted basement membrane （Matrigel）. Results：Oxymatrine could inhibit Ishikawa cell＇s proliferation as reveled by CCK-8 assay. Ishikawa cells viability in presence of oxymatrine decreased markedly in a dose and time-dependent manner. IC50 was 8.07,4.62,3.22mg/ml at 24 hour,48hour and 72 hour respectively. Flow cytometry analysis proved that cell population in G1 phase was increased and cell population in S phase was decreased following Oxymatrine treatment. Oxymatrine can induce apoptosis significantly,and when the concentration was 10mg/ml,the apoptosis rate could reach 65.4%. Oxymatrine also significantly suppressed the invasion of tumor cells to basement membrane. Conclusion： Oxymatrine inhibited the proliferation of Ishikawa cells effectively and induced apoptosis. Oxymatrine is likely to be an promising medicine of treating the endometrial cancer.