摘要
根据GenBank已发表的鸡γ干扰素cDNA基因序列设计一对引物,以血淋巴细胞提取的总RNA为模板,通过RT-PCR的方法克隆出鸡γ干扰素基因,把它与融合表达载体pET32a相重组。通过对阳性宿主菌的不同时间的诱导摸索出最佳表达时间。
A pair of primers specific to chicken γ- interferon gene were designed and synthesized according to the known sequences from GenBank, using the total mRNA in the lymphocyte in chicken blood as the template,the mature protein gene of γ -interferon was cloned and amplified through the reverse transcription polymerase chain reaction. The gene was then ligated to pET32a to establish the gene＇ s non - fusion expressive vector. The best inducing time for the expression of this recombinant protein was tested through inducing the expression of selected positive plasmid with different time span
出处
《江西农业学报》
CAS
2009年第4期90-91,94,共3页
Acta Agriculturae Jiangxi
关键词
鸡γ干扰素
克隆
原核表达
基因
Chicken γ- interferon gene
Gene cloning
Expression
