8种药物对温氏附红细胞体的体外杀灭效果

Drug Screening Against E. wenyoni in Vitro

为确定药物对温氏附红细胞体病的体外杀灭效果,在70 mL RPMI-1640培养液中添加30 mL新生牛血清,2.0 g葡萄糖,0.20 g NaHCO3,37℃,体积分数为0.05的CO2条件下,进行了咪唑苯脲、磷酸伯氨喹、盐酸多西环素、敌百虫、延胡索酸泰妙菌素、依沙吖啶、三氮眯、大蒜素注射液等8种药物对温氏附红细胞体的体外药物杀灭试验。结果表明,咪唑苯脲、磷酸伯氨喹的杀灭效果最好,且在有效浓度范围内对红细胞的影响较轻微;敌百虫、依沙吖啶的杀灭效果也很好,但是对红细胞的损伤较咪唑苯脲和磷酸伯氨喹重。因此,治疗温氏附红细胞体病应首选咪唑苯脲或磷酸伯氨喹,其次为敌百虫和依沙吖啶。

Under the conditions of 37 ℃, φ=0.05 CO2, drug screening was conducted through cell culture with the medium of 70 mL RPMI-1640 which contained 30 mL NBCS, 2. 0 g glylose and 0. 20 g NaHCO3. The results in vitro indicated thai the killing efficacy of imidocarb and primachin phosphate were the best, and they had less influence on erythrocytes than dipterex, ethacridine and etc. There- fore, imidocarb or primachin phosphate should he the first choice to treat eperythrozoonosis, while dipterex and ethacridine are the second choice.