纳米Zn/Al-水滑石对Hela细胞中的ROS及SOD的影响

Effect of Nanometer-sized Zn/Al-hydrotalcite particles on ROS and SOD of Hela cells

目的探讨纳米Zn/Al-水滑石颗粒对Hela细胞的氧化作用。方法采用不同浓度的纳米Zn/Al-水滑石颗粒(50、100、200、400、800μg.ml-1)对Hela细胞进行12h染毒,染毒后对其活性氧(ROS)和超氧化物歧化酶(SOD)含量进行测定。结果不同浓度的纳米Zn/Al-水滑石染毒组细胞与对照组细胞比较,细胞中ROS水平随纳米水滑石浓度的增加有不同程度的升高。纳米水滑石浓度较低时(50μg.ml-1),ROS值与对照组比较无显著性差异(P>0.05);浓度较高时(100、200μg.ml-1)纳米水滑石的ROS值与对照组比较有显著性差异(P<0.05);当纳米水滑石浓度为400、800μg.ml-1时,其ROS值与对照组比较有极显著性差异(P<0.01)。随着纳米水滑石浓度的逐渐升高,SOD水平则有不同程度的降低。纳米水滑石浓度较低时(50、100μg.ml-1),SOD值与对照组比较无统计学意义(P>0.05);浓度较高时(200、400、800μg.ml-1)纳米水滑石与对照组比较有极显著性差异(P<0.01),SOD值显著降低。结论纳米Zn/Al-水滑石可抑制Hela细胞内超氧化歧化酶活性,使过氧化物增多。

Objective To explore and compare the oxidative damage induced by Nano Zn/Al-LDH. Methods Hela cells were exposed to this particle with different concentrations （50,100,200,400,800 μg·ml^-1 ） for 12 h. After exposure ,the contents of reactive oxygen species （ROS） and superoxide dismatase （SOD） in Hela Cells were measured of oxidative damages. Results ROS contents in different concentrations of Nano Zn/Al-LDH were higher than that of saline group. Low concentration （ 50 μg·ml^-1 ） namo Zn/Al-LDH had no significant toxicity to Hela cells （ P 〉 0.05） . While in high concentration, nano Zn/Al-LDH （100, 200 μg·ml^-1） had significant toxicity to Hela cells （P 〈 0.05 ）. With the increase of the concentration, they were much higher than saline group （P 〈 0. 01 ）. Meanwhile, SOD contents in all the groups were lower than saline group. Low concentration （ 50,100μg·ml^-1 ） namo Zn/Al-LDH had no significant toxicity to Hela cells （P 〉 0. 05 ）. While in high concentration, nano Zn/Al-LDH groups （200,400,800μg·ml^-1 ） , were much lower than control group （ P 〈 0. 01 ）. Conclusion Nano Zn/Al-LDH may inhibit the activity of SOD and induce the proliferation of peroxide.