细胞染色体检测在诊断恶性多浆膜腔积液中的临床研究

Clinical study on chromosome detection in the diagnosis of malignant polyserositis

目的探讨细胞染色体检测对诊断恶性多浆膜腔积液的价值。方法染色体检测采取涂片染色镜检定性的方法。本研究共有43例恶性肿瘤和30例非恶性肿瘤伴多浆膜腔积液的患者,对其浆膜腔积液均进行了染色体、癌细胞、CEA等检查。结果浆膜腔积液染色体检测的敏感性为79.07%,准确性为92.65%,明显优于癌细胞、CEA的检测结果(P<0.05,P<0.01),且特异性高达96.67%。31例恶性肿瘤伴渗出性多浆膜腔积液患者中,28例染色体阳性,阳性检出率为90.32%;12例恶性肿瘤伴非渗出性多浆膜腔积液患者中,6例染色体阳性,阳性检出率为50.00%,两组阳性率均较高,但经比较P<0.05。结论恶性肿瘤患者伴发多浆膜腔积液时,无论是渗出液还是非渗出液,其染色体阳性检出率和特异性都很高,对诊断恶性多浆膜腔积液具有很好的临床实用价值,但非渗出液的细胞培养时间应适当延长。

Objective To study the diagnostic value of malignant polyserositis with chromosome. Methods The chromosome were measured with microscope after smear and stain. The chromosome, cancer cell,CEA were detected in all the 43 tumor patients and all the 30 non-tumor patients with polyserositis. Results The sensitivity of the chromosome detection was 79. 07%, the accuracy was 92. 65%, they showed statistically significant superior versus the cancer cell, CEA detection （ P 〈 O. 05, P 〈 0. 01 ） ; and the specificity was 96. 67% o In the percolate of 31 tumor patients,28 results of patient＇s chromosome detection were positive, the positive rate was 90. 32% ;In the non-percolate of 12 tumor patients,6 results of patient＇s chromosome detection were positive, the positive rate was 50. 00% ;Both of them had higher positive, but they showed statistically significant differences too,P 〈 0. 05. Conclusions The results of chromosome detection had better sensitivity and specificity, not only in the percolate of tumor patients but also in the non-percolate of tumor patients. It is more useful in diagnosis of the malignant polyserositis, but it should be longer periods with the breeding cell in the non-pereolate of tumor patients.