铅暴露对雄性黑斑蛙精子毒性和血清性激素水平的影响

Effects of lead exposure on sperm toxicity and levels of sexual hormone in sera of male Rana nigromaculata

在实验条件下,将健康性成熟雄性黑斑蛙暴露于0.1、0.2、0.4、0.8和1.6mg·mL-1浓度的铅溶液中30d,观察和分析黑斑蛙的精子数量、精子活动度和精子畸形率,采用酶联免疫吸附法检测血清中睾酮(T)和雌二醇(E2)的水平,以探讨铅对雄性黑斑蛙的生殖内分泌毒性.结果表明,随铅染毒剂量的升高,精子数量下降,精子畸形率呈剂量依赖性增加,精子活动率呈剂量依赖性降低;随铅染毒剂量的升高,血清中T水平明显下降,E2水平则明显升高,且均呈剂量-效应关系.结果提示,铅对雄性黑斑蛙有明显的生殖毒性,可改变血清睾酮和雌激素水平,干扰生殖内分泌调节平衡.

To evalutate the effects of lead （Pb） exposure on sperm toxicity and levels of sexual hormone in sera of male Rana nigromaculata and to explore the mechanism of lead on male reproductive toxicity, healthy adult male frogs were exposed to 0.1, 0.2, 0.4, 0.8 and 1.6 mg· L ^- 1 of lead solution for 30 days under experimental conditions. The sperm quantity, sperm activity and sperm deformity of the frogs were recorded and analyzed, and the levels of testosterone （T） and estradiol （ E2 ） in sera were measured by enzyme linked immunosorbent assay （ ELISA）. The results showed that the sperm quantity decreased as Pb concentration increased, and Pb caused an increase in percentage of sperms with deformity and a decrease in sperm motility in a dosedependent manner. In addition, the T level of sera reduced while the E2 level increased significantly with Pb concentration, displaying an obvious concentration-effect relationship. These results show that Pb has obvious reproductive toxicity on male Rana nigromaculata and can change tbe testosterone and estradiol levels, and interfere with the reproductive endocrine systems.