蒙药呼和嘎日迪-9多糖的提取与含量测定

Determination of Polysaccharide in Huhegaridi-9

采用热水提取-乙醇沉淀的方法从蒙药呼和嘎日迪-9中提取多糖,分光光度法测定糖含量,求得校准曲线的回归方程为y=0.00656x+0.324,相关系数为r=0.9994,平均回收率为101.2%,RSD=0.90%(n=5)。

Polysaccharide was extracted from huhegaridi-9 with hot water-ethanol deposit method and determined by phenol-sulphuric acid method（λ=488nm）.The regression equation is y=0.00656x＋0.324 and coefficient of correlation r=0.9994.The average recovery is 101.2% with RSD=0.90%（n=5）.