摘要
目的建立人偏肺病毒(hMPV)感染小鼠模型,了解病毒肺内复制规律及所致病理改变,为hMPV感染免疫病理机制研究及新型防治手段开发奠定基础。方法BALB/c小鼠经滴鼻感染荧光标记的重组hMPV,于感染后1、3、5、7、9、16d处死小鼠并无菌获取肺组织用于病毒分离和病理检查,改良噬斑形成法检测hMPV滴度,RT—PCR法检测hMPVmRNA表达。结果小鼠滴鼻感染hMPV后肺组织分离到病毒;肺组织病毒滴度在感染后5d达到高峰(5.16±1.09)×10^5PFU/g,感染后第9天仍能检测到病毒(2.79±1.22)×10^2PFU/g;感染后16d肺组织仍可检测到hMPV mRNA;病理改变在感染后3~7d最明显,为典型的间质性肺炎改变。结论hMPV感染BALB/c小鼠模型建立成功,可用于hMPV感染的免疫病理机制研究。
Objective To establish a mouse model for human metapneumovirus (hMPV) infection, and to explore the course of viral replication and pathology and lay ground for vaccine development. Methods BALB/c mice were infected intranasally with hMPV, and sacrificed on day 1,3, 5,7, 9 and 16 post inoculation. Lungs were used for viral isolation, viral titration by an improved plaque assays, pulmonary histopathology and detection of hMPV mRNA expression by RT-PCR. Results Live viruses were successfully isolated from the lungs of infected mice, hMPV replicate well in the lungs and viral titers peaked on the 5th day post inoculation (5.16 ± 1.09) ×10^5 PFU/g, remaining to be detectable on the 9th day post inoculation (2.79 ± 1.22) ×10^2 PFU/g. Genomic RNA of hMPV was able to be detected on the 16th day post inoculation by RT-PCR targeting F gene. Histopathology of lungs was characterized by interstitial pneumonia in particular during 3-7 days post inoculation. Conclusion hMPV infected BALB/c mice model was successfully established, hMPV infected mice showed similar patterns of viral replication and pulmonary histopathology as in infected humans.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第4期312-315,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金重点项目(30730098),面上项目(30800972)
教育部新世纪优秀人才支持计划(NCET-05-0774)
重庆市杰出青年科学基金(CSTC,2008BA5040)