滋阴泻火方对环境内分泌干扰物染毒大鼠拟雌激素活性的拮抗作用

Antagonistic Effect of Ziyin Xiehuo Recipe on Estrogen-like Activity of Environmental Endocrine Disruptors Exposed Rats

目的观察滋阴泻火方对环境内分泌干扰物壬基酚(NP)及双酚A(BPA)的拟雌激素活性的拮抗作用。方法30只3周龄雌性SD大鼠随机分为对照组(喂饲玉米油),染毒A组(喂饲NP100mg/kg),染毒B组(喂饲NP50mg/kg+BPA200mg/kg),治疗A组(喂饲NP100mg/kg+滋阴泻火方),治疗B组(喂饲NP50mg/kg+BPA200mg/kg+滋阴泻火方),疗程15天,检测子宫增重试验及增殖细胞核抗原(PCNA)蛋白表达。结果染毒A、B组与对照组比较子宫湿重、子宫脏器系数、子宫内膜及平滑肌厚度、子宫内膜腺体及腺上皮高度显著增加(P<0.05),子宫内膜及肌层的PCNA蛋白表达显著增高(P<0.05)。治疗A、B组分别与染毒A、B组相比,上述各指标均明显降低(P<0.05)。结论壬基酚及双酚A具有显著的拟雌激素活性,滋肾阴泻相火中药对壬基酚及双酚A的拟雌激素活性具有显著的拮抗作用。

Objective To explore the antagonistic effect of Ziyin Xiehuo Recipe（ZYXHR, Recipe for nourishing kidney yin and purging ministerial fire） on estrogen-like activity of environmental endocrine disruptors, nonylphenol （NP） and mixture of NP and bisphenol A（BPA）. Methods Totally 30 three-week-old female SD rats were randomly divided into control group（fed with corn oil）, exposed group A （feeding NP100 mg / kg ）, exposed group B （feeding NP 50 mg/kg＋ BPA 200 mg/kg ）, treatment group A （NP 100 mg/kg ＋ ZYXHR）, treatment group B （NP 50 mg/kg＋BPA 200 mg/kg ＋ ZYXHR）. After 15 days of treatment, the uterine weight gain and PCNA protein expression were detected. Results As compared with those of the control group, Uterine wet weight, uterus organ coefficient, thickness of endometrium and smooth muscle, and the height of endometrial glands and glandular epithelium significantly increased in exposed group A and B （P〈0. 05）, PCNA expression in endometrium and myometrium significantly in- creased as well （P〈0.05）. Above indictors were significant decreased in treatment group A and B as compared with those in exposed group A and B （P〈0.05）. Conclusion NP and BPA have estrogenic-like activity,ZYXHR can be antagonistic to the estrogenic effect of NP and BPA.