摘要
目的:探讨复方斑蝥注射液对大肠癌LoVo细胞生长抑制及诱导凋亡的影响。方法:四甲基偶氮唑盐(MTT)测定药物对LoVo细胞抑制作用与时间和剂量的关系,Annexin V-FITC方法对细胞凋亡进行检测,流式细胞术(FCM)检测细胞Fas、Bcl-2蛋白表达水平结果:MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。实验范围内其细胞凋亡成时间与剂量依赖关系。结论:复方苦参注射具有直接的抗肿瘤作用。
Objective：To investigate the effect of cantharides infections on LoVo cell. Methods：The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium. Annexin V-FITC were applied to study cell apoptosis of LoVo cells. FCM were applied to detect the expressions of the Fas and Bel-2 protein. Results：Cantharides iniections could inhibit the proliferation of LoVo cells and could eouse cell apoptosis. Conclusions： Cantharides injections has direct anticancer effect.
出处
《中国医药导刊》
2009年第4期627-629,共3页
Chinese Journal of Medicinal Guide
关键词
大肠癌
斑螫注射液
细胞凋亡
Colon carcinoma
Apoptosis
Cantharides injections
