EphB4受体及其配体EphrinB2 mRNA在子宫内膜癌组织中的表达及意义

The expressions and Significance of EphB4 Receptor and Its ligand phrinB2 mRNA in Endometrial Carcinoma

目的:探讨红细胞生成素诱导的肝细胞受体EphB4及其配体EphrinB2 mRNA在子宫内膜癌组织中的表达及意义。方法:采用逆转录PCR(RT-RCR)法检测33例子宫内膜癌和10例正常子宫内膜组织中EphB4、EphrinB mRNA的表达率及相对含量,并分析二者与子宫内膜癌临床病理特征的关系。结果:(1)EphB4 mRNA在子宫内膜癌组的表达率和相对含量均高于正常子宫内膜组(P<0.05,P<0.05)。EphrinB2mRNA在于宫内膜癌组的表达率和相对含量也均高于正常子宫内膜组(P<0.05,P<0.01)。(2)EphB4、EphrinB2mRNA的表达与子宫内膜癌手术病理分期、组织学分级有密切关系(P<0.05)。二者表达与子宫内膜癌不同组织学类型,肌层浸润程度以及有无淋巴结转移无关(P>0.05)。结论:EphB4和EphrinB2的异常表达与子宫内膜癌发生、进展以及恶性程度有关,二者表达趋势呈现一致性,检测EphB4/EphririB2有助于判断子宫内膜癌预后。

Objective：To study the differential expression of EphB4,EphrinB2 mRNA in endometrial cancer,and to anylaze their significance. Methods：RT-PCR method was used to detect the expression of EphB4,EphrinB2 mRNA in 33 cases of endometrial cancer and 10 cases of nomal endometrial tissues. Results ： （ 1 ） The expression rates and relative content of EphB4 mRNA in endometrial carcinoma were higher than that in normal endometrial tissues significantly. （ P 〈 0. 05, P 〈 0. 05 ）. The expression rates and relative content of EphrinB2 mRNA in endometrial carcinoma were higher than that in normal endometrial tissues significantly. （ P 〈 0. 05, P 〈 0. 01 ）. （ 2 ） The expressions of EphB4 , EphrinB2 mRNA were correlated with their stage and histological grade（ P 〈 0.05 ）. But their expressions had no relationship with the histological type,depth of myometrium invasion and lymphatic metastasis of endometrial carcinoma（ P 〉 0. 05 ）. Conclusions： Overexpression of EphB4 and EphrinB2 may play an important role in the oncogenesis, progression and malignant degree of endometfial cancer. The mRNA expressions of EphB4 and EphrinB2 in endometrial carcinoma were coincident. Detection of EphB4 and EphrinB2 expression might be helpful to predict prognosis of patients with endometrial carcinoma.