脑创伤性鼠红细胞免疫功能的变化

Changes of erythrocyte immunity function in rats with traumatic brain injury

目的:探讨创伤性脑损伤(Traumatic brain injury,TBI)大鼠红细胞免疫功能(Erythrocyte immunity function,EIF)的改变及其机制。方法:选取Wistar大鼠70只,随机分成对照组(n=10)与创伤组(n=60),创伤组制备成轻型(n=30)和中重型(n=30)创伤性脑损伤模型,酵母菌花环法检测对照组与创伤组大鼠血液中红细胞C3b受体花环率(RBC-C3bRR)和红细胞免疫复合物花环率(RBC-ICR),放射免疫法测定血清中β-内啡肽(β-EP)水平。结果:与对照组比较,脑创伤大鼠RBC-C3bRR明显下降(P<0.05),RBC-ICR和β-EP均明显升高(P<0.05),中重型创伤组各指标改变较轻型创伤组更明显(P<0.05)。脑创伤大鼠β-EP变化与RBC-C3bRR呈直线负相关(轻型组r=-0.698,P<0.05;中重型组r=-0.744,P<0.05);β-EP变化与RBC-ICR呈直线正相关(轻型组r=0.835,P<0.05;中重型组r=0.729,P<0.05)。结论:大鼠脑创伤后红细胞免疫功能明显下降,其改变与体内β-EP明显升高密切相关。

Objective： To explore the changes and mechanism of Erythrocyte Immunity Function（EIF） in rats with Traumatic Brain Injury （TBI）. Methods： Healthy Wistar rats （n=70）were divided into control group （n= 10 ）and TBI group （n=60 ）, model of TBI were prepared and divided into mild group （ n=30 ） and moderate to severe group （n=30）. Red Blood Cell-C3b Receptor Rate （ RBC-C3bRR ） and Red Blood Cell-Immune Complex Rate （ RBC-ICR ） of rats with TBI were measured by methed of yeast fungus and the content of serum -endophin （ β -EP） was tested by radioimmunoassay. Results ： Compared with the control group, RBC-C3bRR of rats with TBI decreased significantly while RBC-ICR and β-EP increased significantly（P〈0.05 ）.Moreover, the moderate to severe group had more manifest difference than the mild group（P〈0.05 ）. β-endorphin was negatively correlated with RBC-C3bRR（r=0.698 ,P〈0.05 ）and positively with RBC-ICR（r=0.744, P〈0.05 ） in mild group;and negatively correlated with RBC-C3bRR（r=-0.835, P〈0.05 ）and positively with RBC-ICR （r=0.729 ,P〈0.05 ）in moderate to severd group. Conclusion： ElF in rats with TBI decreased significantly which demonstrated erythrocyte immune dysfunction was related with increasing of the β -EP.