hsa-miR-518b反义寡核苷酸对胃癌细胞生长抑制作用的研究

Effects of antisense oligonucleotide targeting hsa-miR-518b on the growth of gastric cancer cell

目的:探讨hsa-miR-518b反义寡核苷酸(Antisense oligonucleotide,ASODN)对胃癌细胞生长和细胞周期的影响,以分析其在胃癌中的作用机制。方法:设计合成全硫代磷酸化修饰的hsa-miR-518b ASODN,将其转染于胃癌细胞株SGC7901。用Realtime RT-PCR分析hsa-miR-518b在胃癌细胞转染前后的表达情况,MTT检测胃癌细胞转染后的生长情况,流式细胞仪分析细胞周期。结果:转染hsa-miR-518b ASODN后,胃癌细胞hsa-miR-518b表达显著降低(P=0.000),生长受到抑制(P=0.000),大量胃癌细胞阻滞于G1期(P=0.000)。结论:hsa-miR-518b ASODN能使胃癌细胞阻滞于G1期,抑制胃癌细胞的生长。提示hsa-miR-518b可能通过促进细胞周期G1/S期转换而促进胃癌细胞生长。

Objective：To investigate the effects of antisense oligonucleotide（ASODN ） targeting hsa-miR-518b on the growth and cycle of gastric cancer cell, and the mechanism in gastric cancer. Methods： Specific phosphorothioate antisense oligodeoxynucleotides targeting hsa-miR-51gb were synthesized and then transfected into the gastric cancer cell（ SGC7901 ）. We investigated the change of the hsa-miR-518b expression in SGC7901 after the transfection by using real time RT-PCR.Methyl thiazolyl tetrazolium （MTT）assay was used to detect the cell growth of SGC 7901 and fluorescence activated cell sorting （FACS）was used to analyze the cell cycle of SGC7901. Results ： After transfection, the expression of hsa-miR-518b was decrased （P=0.000）, the growth of SGC7901 was suppressed （P=-0.000） and the cell cycle arrested in Gl （P=0.000）. Conclusion： hsa-miR-518b （ASODN）could suppress the growth of SGC 7901 by arresting the cell cycle in G1 phase. Hsa-miR-518b could promote the growth of gastric cancer cell by regulating C1/S transition.