大肠埃希菌Ⅰ类整合子与耐药性分析

Relationship Between Class Ⅰ Integron and Drug Resistance in Escherichia Coli

目的:了解Ⅰ类整合子在临床分离大肠埃希菌和健康人群大肠埃希菌中分布流行状况。方法:根据NCCLS推荐的琼脂稀释法进行药敏试验;PCR扩增Ⅰ类整合酶基因。结果:患者大肠埃希菌Ⅰ类整合子检出率为45.4%;健康人大肠埃希菌Ⅰ类整合子检出率为5.4%。108株患者大肠埃希菌对12种抗菌药物耐药率,最高为氨苄西林97.2%,最低为亚胺培南0.93%。结论:Ⅰ类整合子在患者大肠埃希菌中的存在已经相当普遍,健康人群大肠埃希菌也携带有Ⅰ类整合子,应加强基因水平耐药监测。

Objective：To investigate the occurrence and distribution of class Ⅰ integrase gene among Escheriehia coli isolates from clinical patients and healthy people. Methods ：Characters were examined for their sensitivity to antibiotics by agar dilution test according NCCLS. The presence of class Ⅰ integron was extended by PCR. Results ：The positive rate of class Ⅰ integron was 45.4% in clinical isolates and 5.4% in healthy people isolates respectively. The resistance ratio of 108 strains of Escherichia coli from clinical patients towards 12 antibiotics was between 97.2% and 0.93%. Conclusions ：It is very universal that class Ⅰ integron exists in clinical Escherichia coli. Class Ⅰ integron has been identified among Escherichia coli isolated from healthy people. The results stresses the need for surveillance of bacteria in genetic levels.