系统性红斑狼疮患者外周血单个核细胞周期变化的初步研究

Alteration of Cell Cycle-Phase in Peripheral Blood Mononuclear cells in Patients with Systemic Lupus Erythematosus

目的:探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)细胞周期的变化。方法:采用流式细胞仪检测SLE患者PB-MCs植物血凝素(phytohemagglutinin,PHA)刺激前后细胞周期变化,并与正常人比较。结果:PHA刺激前,SLE患者PBMCs细胞周期构成与正常人无明显差异;刺激后SLE患者PBMCs G0/G1期比例降低,S期比例增高,与正常人比较差异有统计学意义。结论:SLE患者PBMCs较正常人易于活化及增殖。

Objective：To study proliferation activity of peripheral blood mononuclear cells of patients with systemic lupus erythematosus, we observed distribution patterns of cell cycle - phase in these cells. Methods： Distribution patterns of different cell cycle - phase in patients and controls were measured by flow cytometry with or without stimulation by PHA. Results：There were no significant difference between patients and controls in the distribution of different cell cycle - phase at base line without stimulation. Whereas, after the samples were stimulated with PHA, the proportion of phase G0/G1 decreased and the phase S increased in cell samples from SLE patients. Conclusion：There were strong proliferation activity in PBMCs of patients.