氯化钾对大鼠平滑肌细胞硫化氢生成及超微结构的影响

Effect of Chloratum Kalium on the Production of Hydrogen Sulfide and Ultramicrostructure in Pulmonary Artery Smooth Muscle Cells

目的探讨氯化钾（KCl）对大鼠平滑肌细胞硫化氢（H2S）生成及其对超微结构的影响。方法取大鼠胸主动脉平滑肌细胞（SMCs）进行体外培养，分为对照和不同时间点的KCl组。每组取4个样品，细胞密度控制在（1～2）×10^5／L。按50mol／L的浓度加入KCl溶液，在第5，15，和30分钟分别吸取每个样品5ml，低速离心，用敏感硫离子电极测量每个样品培养基中所含的硫离子浓度；所获细胞进行透射电镜观察分析。结果第5，15和30分钟KCl组与对照组相比，细胞培养基中硫离子浓度分别增加162．72％（P〈0．05），253．68％（P〈0．01），63．23％（P〈0．05）；各KCl组的细胞内粗面内质网较生理盐水对照组丰富。结论KCl能促进平滑肌细胞合成H2S。

Objective To explore the effect of chloratum kalium（ KCl）on the production of H2S and ultramicrostructure of pulmonary artery smooth muscle cells（ PASMCs）. Methods Rat＇s cultured PASMCs were randomly di- vided into control group and KCl groups at different time poits. Each group contained 4 samples （ V = 5ml, D = 1 - 2 ×10^5/L）. The KCl was added into PASMCs at the concentration of 50mol/L. 5ml of each sample was sucked at the 5th, 15th and 30th minute. The concentration of sulfion was measured by sensitive sulfion electrode and the cultured PASMCs were observed by electron microscope. Results Compared with the control group,the concentration of sulfion was respectively increased by 162.72% （ P 〈 0.05 ） ,253.68% （ P 〈 0.01 ） ,63.23% （ P 〈 0.05 ） for PASMCs in each KCl group ; the number of rough endoplasmic reticulum （RER） in KC1 group is much more than that in control group. Conclusion KCl can sublimate the concentration of sulfion in rat＇s PASMCs.