非小细胞肺癌中NOK蛋白表达的临床意义

The clinic significance of NOK protein expression in NSCLC

目的:检测NOK蛋白在非小细胞肺癌中的表达,分析其表达率与病理分型、分级及临床TNM分期间的关系,为研究NOK的功能及其机制提供临床证据。方法:采用免疫组织化学EnVinsion法检测NOK蛋白在非小细胞肺癌(NSCLC)中的表达。结果:免疫组化结果显示NOK阳性表达主要位于胞质中。NSCLC中NOK总阳性率68.1%,癌旁组织阳性率只有12.1%,差异性非常显著(P=0.000);肺鳞癌和腺癌总阳性率分别为60.49%、78.33%,两者差异显著(P=0.009);鳞癌中分化、低分化阳性率分别为51.1%、72.22%,差异显著(P=0.01),腺癌的高、中、低分级的阳性率分别是20.0%、79.5%、100%,经Kruskal-WallisH检验差异性非常显著(P=0.001);NSCLC(鳞癌、腺癌)TNM分期Ⅱa、Ⅱb、Ⅲa、Ⅲb的阳性率,经Kruskal-WallisH检验其差异性非常显著(P=0.000)。结论:NOK在肺鳞癌、腺癌中高表达,表达的高低与病理分型、分级及临床TNM分期(转移)有关。

Objective：To investigate the expression of NOK protein, and analyze the relationship between NOK expression and pathological types, grades and clinical stages （TNM stages） in the non small cell lung cancer （NSCLC）. Methods：Samples from 141 cases with NSCLC were collected to detect the expression of NOK protein by immunohistochemical staining （EnVinsion）. Results：The NOK positive expression was mainly located in the cytoplasm. NOK positive staining was 68.1% in NSCLC ,while the positive rate of the same samples adjacent tissues was 12.1% （ P =0. 000）. Samples with NOK positive staining were observed to cover 60.49% and 78.33% in the squamous cell lung cancers and the lung adenocarcinomas respectively （P =0.1309）. The positive NOK staining were 51.1% and 72.22% in the moderately differentiated and low differentiated squamous cell lung cancers respectively （P = 0.01 ）, while the positive staining covers 20.0% ,79.5 % , and 100% in the highly, moderately and low differentiated lung adenocarcinoma respectively （P = 0.001 ）. Significant differences of the NOK positive staining among different TNM stages in NSCLC were also observed by Kruskal - Wallis test （ P = 0. 000）. Conclusion： The NOK protein level is elevated in NSCLC, and the expression is correlated with the pathological type, grade and TNM stage in NSCLC.