应用聚合酶链反应技术鉴定印度腥黑穗病菌

The identification of Tilletia indica using PCR

用一对专化于印度腥黑穗病菌的引物Ｔ１１７Ｍ１（５＇－ＴＣＣＣＣＴＴＧ－ＧＡＴＣＡＧＡＡＣＧＴＡ－３＇）和Ｔ１１７Ｍ２（５＇－ＡＧＡＡＧＴＣＴＡＡＣＴＣＣＣＣＣＣＴＣＴ－３＇）可特异地扩增印度腥黑穗病菌产生一段８２５ｂｐ的产物，而稻粒黑粉病菌则不能被扩增。实验还表明，用聚合酶链反应（ＰＣＲ）方法检测灵敏度可达到１００个未萌发的冬孢子，这为进口粮印度腥黑穗病菌的检疫提供了有力工具。

A pair of primer T117M1 (5' TCCCCTTGGATCAGAACGTA 3') and T117M2 (5' AGAAGTCTAACTCCCCCCTCT 3') could specially amplify a fragment of 825 bp from Tilletia indica DNA and no amplity from Tilletia barclayana DNA. The result also shows that DNA extracted from 100 ungerminated teliospores of Tilletia indica can be detected by PCR, and PCR is a useful method in the quarantine of Tilletia indica in import cereal.