鸡球虫弱毒虫株的临床前应用

EXPERIMENT OF APPLICATION OF ATTENUATED EIMERIA PRIOR TO THE FIELDS

以不同剂量的柔嫩艾美耳球和巨型艾美耳球虫的弱毒虫株免疫7日龄雏鸡，测定虫株的最小有效免疫剂量、免疫力产生时间、免疫有效期及虫株的保存期。结果表明，柔嫩艾美耳球虫和巨型艾美耳球虫的弱毒虫株最小有效免疫剂量分别为500个卵囊／鸡和200个卵囊／鸡。免疫后一周即可产生坚强的免疫力，免疫有效期达8周以上。弱毒虫株在4℃环境中的有效保存期为7个月。

in this study, 7-day old chickens were orally immunized with attenuated E. tenella and E. maxima at different dosages. The least effective immune dosage,immunity-producing time,effective immune period and preserving period were determined. The results showed that,the least effective immune dosages of E.tenella and E. maxima were 500 and 200 oocysts per chicken respectively,the strong immunity was obtained 1week post inoculation,the effective immune period was more than 8 weeks and the preserving period was upto 7 months at 4℃.