硼对大豆愈伤组织的细胞结构及形态的影响

Effect of Boron on Cell Structure and Shape in Soybean(Glycine max) Callus

硼是植物生长发育必需的微量元素之一,对维持植物细胞壁与细胞膜的结构和功能发挥起着重要的作用。在对大豆愈伤组织诱导培养技术成熟的基础上,研究高浓度的硼对大豆愈伤组织的细胞结构和细胞形态的影响,探讨组织培养中硼对愈伤组织诱导与再生的调节作用。采用植物组织培养的方法,在硼酸含量不同的培养基上诱导大豆下胚轴产生愈伤组织,并进行继代培养试验。结果表明:下胚轴在硼酸浓度大于70mg·L-1的培养基上诱导20d后,一些新生的愈伤组织结构疏松,细胞分散,细胞间的正常连接无法形成。把这些结构疏松的愈伤组织在正常的培养基MS+2,4-D0.4mg·L-1上进行继代试验,发现细胞仍具有分裂能力,新生的愈伤组织细胞结构更加松散,单细胞的数目增多,并且细胞的形态也各异。

Boron is one of the essential microelements for plant growth, playing an important role in maintaining the structural and functional integrity of cell wall and cellular membrane. In this experiment the influences of excess boron on structure and shape of callus cells and the regulatory, function of boron in callus induction and regeneration were studied using the successfully induced soybean callus. The medium with various content of boric： acid were used to culture soybean tissue of hypocotyledonary axis to induce the callus and successive cell generations. Our results suggested that some callus on the medium with over 70 mg · L^-1 boron was loosen and its cells were rarefaction after twenty day- culture. When this callus was transterred into the normal medium MS ＋ 2,4- D 0.4 mg · L^-1 for a subsequent culture, it was observed that the cells of the callus still had the capability of division and the structure of the callus became looser and more unicells appeared with various cell shapes.