用聚乙二醇沉淀法筛查高泌乳素血症的应用评价

Evaluation of polyethylene glycol precipitation test as a screening method for hyperprolactinemia

目的探讨用聚乙二醇(PEG)沉淀法去除假性高泌乳素(PRL)血症的临床应用评价。方法测定232例高值PRL患者及52名正常体检者血清PRL,血清经PEG沉淀后再次测定其PRL,同时用缓冲液沉淀做对照,并对二者结果进行比较。结果高值PRL组中巨PRL检出率为25.4%,正常对照组中巨PRL的检出率为0.0%,2组差异有统计学意义(P<0.01)。单体PRL组PEG处理前后血清PRL差异无统计学意义(P>0.05),而巨PRL组和可疑巨PRL组PEG处理前后PRL差异有统计学意义(P<0.01)。3组经缓冲液处理前后PRL差异无统计学意义(P>0.05)。结论高值PRL部分是假性升高,用PEG沉淀法筛查高值PRL对提高临床诊断的准确性有一定的价值,能减少对高PRL血症的误诊。

Objective To eliminate false hyperprolactinemia by polyethylene glycol （PEG） precipitation test. Methods Prolactin （PRL） was detected in sera before and after precipitation by PEG from 232 patients with hyperpro- lactinemia and 52 healthy controls. The results of PRL were compared with that of a buffer control group at the same time. Results The positive rate of macroprolactinemia in hypeiprolactin group and healthy controls was 25.4% and 0.0% respectively. The differences between the two groups were statistically significant（P 〈 0.01 ）. Before and after treatment with PEG, the level of PRL in monoprolactin group was not statistically significant （P 〉 O. 05）, while that in the macroprolactin group and the suspicious macroprolactin group were statistically significant （P 〈 0. O1 ）. Before and after treatment with buffer, the serum levels of PRL in three groups were not statistically significant （ P 〉 0.05 ） . Con- clusions There is false hyperprolactinemia. PEG precipitation test is valuable to improve the accuracy of hyperprolactin determination and reduce the misdiagnosis of hyperprolactinemia.