两个与玉米大斑病抗性基因Htn1连锁的RFLP标记的原位杂交定位

CHROMOSOME LOCATION OF TWO RFLP MARKERS UMC84 AND UMC30 TIGHTLY LINKED TO THE Htn1 GENE IN MAIZE

以玉米黄早四自交系为材料，采用生物素标记的染色体原位杂交对与大斑病抗性基因Ｈｔｎ１紧密连锁的两侧２个ＲＦＬＰ标记ＵＭＣ８４和ＵＭＣ３０进行了定位。结果表明：ＵＭＣ８４和ＵＭＣ３０探针都同时与第８和第６染色体杂交，平均信号检出率为１４．７％。ＵＭＣ８４和ＵＭＣ３０在第８号染色体长臂上杂交信号与着丝粒的百分距离分别为３８．２６±１．９７和３７．９２±３．４８。在第６号染色体长臂上杂交信号与着丝粒的百分距离分别为３３．５８±３．２８和３４．９６±２．１３。这些结果说明ＵＭＣ８４和ＵＭＣ３０之间的物理距离相距很近，以此推断ＵＭＣ８４或ＵＭＣ３０的位置所在区域应该是Ｈｔｎ１所处的区域。

A biotin labelled in situ hybridization technique was used to physically map two RFLP markers UMC84 and UMC30 which were tightly linked to the Htn1 gene in the chromosomes of maize.The results showed that two markers were located on the chromosomes 8,6 and the average detection rate of in situ hybridization was 14.7%.The percent distances of UMC84 and UMC30 from centromere on the chromosome 8 were (38.26±1.97)% and (37.92±3.48)% respectively and on the chromosome 6 were (33.58±3.28)% and (34.96±2.13)% respectively.This indicates that there are no difference between genetic and physical distances of two markers UMC84 and UMC30 and the sites of UMC84 or UMC30 should be the location of Htn1.