摘要
以玉米黄早四自交系为材料,采用生物素标记的染色体原位杂交对与大斑病抗性基因Htn1紧密连锁的两侧2个RFLP标记UMC84和UMC30进行了定位。结果表明:UMC84和UMC30探针都同时与第8和第6染色体杂交,平均信号检出率为14.7%。UMC84和UMC30在第8号染色体长臂上杂交信号与着丝粒的百分距离分别为38.26±1.97和37.92±3.48。在第6号染色体长臂上杂交信号与着丝粒的百分距离分别为33.58±3.28和34.96±2.13。这些结果说明UMC84和UMC30之间的物理距离相距很近,以此推断UMC84或UMC30的位置所在区域应该是Htn1所处的区域。
A biotin labelled in situ hybridization technique was used to physically map two RFLP markers UMC84 and UMC30 which were tightly linked to the Htn1 gene in the chromosomes of maize.The results showed that two markers were located on the chromosomes 8,6 and the average detection rate of in situ hybridization was 14.7%.The percent distances of UMC84 and UMC30 from centromere on the chromosome 8 were (38.26±1.97)% and (37.92±3.48)% respectively and on the chromosome 6 were (33.58±3.28)% and (34.96±2.13)% respectively.This indicates that there are no difference between genetic and physical distances of two markers UMC84 and UMC30 and the sites of UMC84 or UMC30 should be the location of Htn1.
出处
《植物病理学报》
CAS
CSCD
北大核心
1998年第2期117-121,共5页
Acta Phytopathologica Sinica
基金
国家自然科学基金
国家教委博士点基金