戊乙奎醚对鼠小肠运动功能的影响

Effect of penehyclidine on small intestinal motility in mice and rats

目的评价戊乙奎醚对鼠小肠运动功能的影响。方法实验Ⅰ：昆明小鼠24只，体重17～22g，雌雄不拘，随机分为3组（n=8）：对照组（C1组）、阿托品组（A1组）和戊乙奎醚组（P1组）。A1组腹腔注射阿托品0．3mg／kg，P1组腹腔注射戊乙奎醚0．3mg／kg，C1组腹腔注射等容量生理盐水。给药后15min以营养性半固体糊0．8ml灌胃行小肠推进实验，灌胃后20min处死，取小肠，测量小肠全长（幽门至回盲部距离）及幽门至营养性半固体糊推进的距离，计算小肠推进百分比。实验Ⅱ：Wistar大鼠24只，体重170～220g，雌雄不拘，随机分为3组（n=8）：对照组（C2组）、阿托品组（A2组）和戊乙奎醚组（P1组）。A2组腹腔注射阿托品2mg／kg，P2组腹腔注射戊乙奎醚2mg／kg，C2组腹腔注射等容量生理盐水。给药后30min取左心室血样，采用放免法测定血浆胃动素浓度。结果与C2组比较，A1组小肠推进百分比降低（P〈0．05），P1组小肠推进百分比差异无统计学意义（P〉0．05）；与A1组比较，P1组小肠推进百分比升高（P〈0．05）。与C2比较，A2组血浆胃动素浓度降低（P〈0．05），P2组血浆胃动素浓度差异无统计学意义（P〉0．05）；与A1组比较，P1组血浆胃动素浓度升高（P〈0．05）。结论戊乙奎醚对鼠小肠运动功能没有明显影响。

Objective To investigate the effect of penehyclidine on small intestinal propulsion in mice and plasma level of motilin in rats. Methods The experiment was performed in 2 parts. In experiment Ⅰ , twenty-four healthy Kumning mice of both sexes weighing 17-22 g were randomly divided into 3 groups （ n = 8 each） ： group Ⅰ control （C1）, group Ⅱ received intraperitoneal （IP） atropine 0.3 mg/kg （A,） and group Ⅲ received IP penehyclidine 0.3 mg/kg （P1）. Nutritional semi-solid cataplasm 0.8 ml was given into the stomach at 15 min after IP injection. The mice were killed 20 min later. The abdomen was cut open. The length of the small intestine and the distance between the pylorus and the nutritional semi-solid cataplasm in the small intestine were measured. The rate of intestinal propulsion was calculated. In experiment Ⅱ , twenty-four Wistar rats of both sexes weighing 170- 220 g were randomly divided into 3 groups （n = 8 each）; group Ⅰ control （C2）; group Ⅱ received IP atropine 2 mg/kg （A2 ） and group m received IP penehyclidine 2 mg/kg （P2） . Blood samples were taken at 30 min after IP drug administration for determination of plasma motilin concentration （by radio-immuno-assay） .Results The rate of small intestinal propulsion was significantly lower in group A1 than in group C1. There was no significant difference in the intestinal propulsion between group C1 and P1. The plasma concentration of motilin was significantly lower in group A2 than in group C2 but there was no difference in plasma motilin concentration between group P2 and C2. The plasma concentration of motilin was significantly lower in group A2 than in group P2. Conclusion Penehyclidine has no effect on small intestine motility.