红色荧光蛋白标记的裸鼠胰腺癌转移模型的建立

Establishment of red fluorescent protein nude mice metastasis models of pancreatic cancer

目的建立稳定、高表达红色荧光蛋白标记的裸鼠胰腺癌转移模型。方法将RFP基因转入人胰腺癌细胞株SW1990。评价转染前后细胞生物学行为有无改变，并采用SW1990-RFP建立实验性肺转移模型和实验性肝转移模型。应用整体荧光成像系统评价各组转移模型肿瘤转移的发生。结果获得稳定，高表达RFP的单克隆细胞系SW1990-RFP，转染前后细胞生物学行为无明显改变。成功建立了裸鼠胰腺癌实验性肺转移模型和肝转移模型，并应用整体荧光成像系统检测到各脏器微小转移的发生，建模成功率均为66．7％（4／6）。结论RFP基因不改变SW1990的细胞生物学行为，本实验所建立的裸鼠胰腺癌转移模型稳定、可靠，具有较强的灵敏性及特异性。

Objective To establish stable high RFP-expressing nude mice metastasis models of pancreatic cancer. Methods RFP gene was transfected into human pancreatic cancer cells SW1990. The biological behavior of the two cell lines was assessed. The experimental lung metastasis model and the experimental liver metastasis model were established by SW1990-RFP. The metastasis of pancreatic cancer ceils was assayed by whole-body fluorescence imaging system. Results We obtained stable high RFP-expressing clone SW1990-RFP. The biological behavior of SW1990 and SW1990-RFP were not clearly different. RFP experimental metastasis models of pancreatic cancer were successfully established with the success rate being 66.7%. The metastasis and micrometastasis of orgins in early stage were detected by using whole-body fluorescence imaging system. Conclusion The REP gene didn＇ t change the biological behavior of SW1990. The RFP metastasis models of pancreatic cancer are stable and reliable.