遮荫对烟草中部叶片腺毛密度及其分泌物含量的影响

Effects of Shaded Treatment on Density of Central Trichome and Content of Glandular Secretions in Tobacco

采用广视野显微镜和GC/MS,分析研究了遮荫条件下及正常光照条件下烟草中部叶片腺毛密度及其分泌物的变化情况。结果表明:随着移栽时间的增加,不同光强条件下烟草叶片尖部、中部、基部腺毛密度都依次降低;遮荫条件下烟草叶尖部、中部、基部的腺毛密度低于正常光照条件,差异达显著水平;腺毛总密度差异达极显著水平。对不同光照条件下烟草叶面浸提物进行GC/MS分析,遮荫条件下烟草腺毛主要分泌物———西柏三烯二醇的含量明显低于正常光照条件下。表明光强对烟草叶面腺毛的发育及分泌活动具有显著影响。

Density of trichome and glandular secretions and changes in circumstances of tobacco central leaf under the conditions of the shade and normal light were analyzed by the method of wide field of vision microscope and GC/MS. The results showed that with the increase in transplanting time and different light conditions,the density of trichome decreased as the order of leaf tip,the middle leaf and basal leaf. The density of leaf trichome under shaded treatment is lower than those under normal light conditions,and achieve a significant level of difference. Throughout the process,the density of trichome under the conditions of shaded treatment were lower than those under normal light conditions and up to a very significant difference. The glandular secre- tions of tobacco leaf were analyzed by the method of GC/MS under different light conditions. The content of the major secretion, leukotrienes glycol duvatriene diol, was significantly lower under shaded treatment than that under the normal light conditions. The light has a significant impact on the development of trichome and excreting of glandular secretion in tobacco leaf.