大豆甙元对去卵巢大鼠骨代谢酶及骨重建的影响

EFFECTS OF DAIDZEIN ON THE ENZYMES OF BONE METABOLISM AND BONE REBUILDING IN OVARIECTOMIZED RATS

目的探讨大豆甙元(daidzein,Daid)改善去卵巢大鼠骨代谢酶学指标及对骨重建的作用。方法60只3月龄雌性清洁级大鼠随机分为:假手术(Sham)、去卵巢(OVX)和4个不同剂量去势+大豆甙元组(OVX-D1～OVX-D4),共6组。Sham和OVX组大鼠每日灌服等量蒸馏水,OVX-D1、OVX-D2、OVX-D3和OVX-D4四组分别灌服25、50、75和100mg/kgbw的Daid。大鼠6个月后处死,收集血液、肝脏和股骨,测定钙、磷含量及骨代谢酶学指标。结果Daid可提高去卵巢大鼠血清Ca、P含量及超微量ATP酶的表达水平,降低血清碱性磷酸酶(ALP)、血清骨特异性抗酒石酸酸性磷酸酶(TRAP-5b),其作用与剂量有关。结论服用一定剂量的Daid,能够抑制骨代谢相关酶的表达,进而抑制去卵巢大鼠的骨形成与骨吸收,使骨吸收与骨形成作用达到新的偶联平衡,其作用机制主要是通过降低骨转换率来实现。

Obejective To study the effect of daidzein （Daid） on the enzymes of bone metabolism and bone rebuilding in ovariectomized rats. Method Sixty 3-mon female SD rats were divided into 6 groups Sham, OVX, OVX-D1 -D4 groups. Every day the rats in Sham and OVX groups were given distilled water, and the rats in OVX-D1, OVX-D2, OVX-D3, OVX-D4 groups were given 25, 50, 75, 100 mg/kg bw Daid respectively. After 6 mon, all of rats were killed, and the blood, liver and femur were collected. The content of calcium and phosphorus in serum and the enzymes of bone metabolism were measured. Results The content of calcium, phosphorus and ATPase in serum was increased, but the activity of serum alkaline phosphatase （ALP） and tartrate-resistant acid phosphatase （TRAP-Sb） was decreased dosedependently in OVX-D groups compared with OVX group. Conclusion The expression of bone metabolism-associated enzymes could be inhibited, which resulted in inhibition of bone formation and absorbsion in ovariectomized rats. The new balance between bone formation and absorption could be rebuiltby Daid, through the mechanism of decreasing rate of bone conversion.