富硒锌益生菌对犬的全血硒锌含量与抗氧化能力和肠道菌群的影响

EFFECTS OF SELENIUM-AND ZINC-ENRICHED PROBIOTICS ON BLOOD CONTENT OF SELENIUM AND ZINC,ANTIOXIDATION FUNCTION AND INTESTINAL MICROFLORA IN CANINES

目的研究富硒锌益生菌对犬全血硒锌含量、抗氧化能力和肠道菌群的影响。方法8条18月龄本地犬,雌雄各半,均分成对照组和试验组。对照组在整个试验期间饲喂犬基础日粮,试验组在饲喂犬基础日粮的基础上,每天补给富硒锌益生菌2.0g,连续30d。分别于饲喂的第0d、15d和30d采样测定全血硒锌含量与抗氧化能力和肠道菌群的相关指标。结果在试验15d,犬全血硒和锌含量、全血GPX活性、血清SOD活性、血清T-AOC和粪中乳杆菌数量显著高于对照组,粪中肠杆菌数量显著低于对照组,血清MDA含量和粪中双歧杆菌、葡萄球菌和肠球菌数量与对照组之间差异不显著;在试验30d,犬全血硒含量、血清SOD活性、血清T-AOC和粪中乳杆菌数量极显著高于对照组,全血锌含量、GPX活性和粪中双歧杆菌数量显著高于对照组,粪中肠杆菌数量极显著低于对照组,血清MDA含量、粪中葡萄球菌和肠球菌数量显著低于对照组。结论富硒锌益生菌能提高犬的全血硒和锌含量,提高机体抗氧化能力,调节肠道菌群和改善肠道内环境。

Objective To investigate the effects of selenium- and zine-enriched probiotics on the content of selenium and zinc in blood, antioxidation function and intestinal microflora in canine. Method Eight 18-month native canines, female and male in half, were randomly divided into the control and treatment groups on average. The control group received basal diet, the treatment group received basal diet supplemented with 2.0g selenium- and zinc-enriched probiotics everyday. To determine the experimental indices, the samples were collected on DO, D15 and D30, respectively. Results Compared with the control group, on D15, the content of selenium and zinc in blood, blood GPX, serum SOD, T-AOC and the amount of Lactobacillus in the experimental group were significantly increased, while the amount of Escherichia coli significantly decreased, but the serum MDA and the amount of Bifidobacterium, Staphylococcus and Enterococcus had no significant change. On D30, the content of selenium in blood, serum SOD, T-AOC and the amount of Lactobacillus were very significantly increased, while the content of zinc in blood, blood GPX and the amount of Bifidobacterium significantly increased; but serum MDA and the amount of Escherichia coli, Staphylococcus and Enterococcus very significantly decreased. Conclusion Selenium- and zinc-enriched probiotics could increase content of selenium and zinc in blood, enhance antioxidation function, improve and regulate the intestinal microflora.