姜黄素对体外培养异位内膜细胞生长及凋亡的影响

Effects of Curcumin on Growth and Apoptosis of Isolated Ectopic Endometrium Cells in Vitro

目的观察体外培养的异位子宫内膜细胞(异位内膜细胞)加入姜黄素后的生长情况及形态学变化,并检测其凋亡率及caspase-3基因表达,探讨子宫内膜异位症(内异症)的药物治疗。方法22例内异症患者的异位内膜离体组织分离为单细胞后进行培养,在相同的生长期加入不同剂量(0、10、50μmol/L)的姜黄素后,观察其生长状况;采用TUNEL法检测细胞凋亡率;S-P免疫组化测定异位内膜细胞caspase-3基因的表达。结果加入姜黄素后,细胞生长速度减慢,其凋亡率较未用药组增加,且呈时间剂量依赖性,caspase-3表达较未用药组增加,具有显著差异。结论姜黄素能明显抑制异位内膜细胞的体外生长;上调caspase-3基因蛋白的表达、诱导细胞凋亡可能是其作用机制之一。

Objective： To explore the effects of cureumin on growth, apoptotie rate and the caspase - 3 gene expression of isolated eetopic endometrium cells in vitro, and to study the drug treatment of endometriosis. Methods： The cells of isolated ectopic endometrium from 22 patients with endometriosis were cultured. In the same growth term, curcumin of different dosage were added. The rates of apoptosis were determined by TUNEL, and the gene expressions of easpase - 3 were determined by S - P immunohistochemistry. Results： The speed of cell growth was significantly decreased, and the apoptotic rate and gene expressions of easpase- 3 were significantly increased compared with no eurcumin. It showed time -dosage dependence. Conclusion： Curcumin can significantly inhibit the growth and proliferation of ectopic endometrium cells in vitro, and the effect maybe related to raising the gene expression of caspase - 3 and inducing apoptosis.