慈菇支链淀粉的分离纯化及其分子结构研究

Study on Separation of Arrowhead Amylopectin and Its Molecular Structure

本研究采用正丁醇法对慈姑支链淀粉与直链淀粉进行分离,Sepharose2B凝胶层析表明支链淀粉得到纯化。采用酶法分析了慈姑支链淀粉的分子结构,结果表明慈姑支链淀粉的平均链长(CL)为22,其中外链长(ECL)为14,内链长(ICL)为7。异淀粉酶和普鲁兰酶依次作用于慈姑支链淀粉的β-极限糊精,计算得支链淀粉A链与B链比值为1.33:1.0,分支化度为2.33。

Amylose and amylopectin of arrowhead starch were successively separated by n-butanol recrystallization. It was proved that the obtained amylopectin was pure by chromatography on Sepharose 2B column. Furthermore, molecular structure of the amylopectin was analyzed by enzymatic method. The results showed that average chain length （CL）, interior chain length （ICL） and exterior chain length （ECL） of the amylopectin are 22, 14 and 7, respectively. β-amylase limit dextrin of the amylopectin were completely debranched by orderly hydrolyzing with isoamylase and pullulanse. The ratio of A chain and B chain is calculated to be 1.33：1.0, and the multiple branching degree of arrowhead amylopectin is 2.33.