大鼠肾移植急性排斥模型中移植肾水通道蛋白-2 mRNA表达的变化

The change of aquaporin-2 mRNA in the graft of acute rejection rat renal transplantation model

目的研究水通道蛋白-2(AQP2)mRNA在大鼠肾移植急性排斥模型中移植肾表达的变化及意义。方法正常大鼠肾脏为正常对照组;Wistar大鼠作为供、受者建立同系基因对照组;SD大鼠为供者、Wistar大鼠为受者建立同种异体移植急性排斥组;SD大鼠为供者、Wistar大鼠为受者,术后给予免疫抑制剂环孢素(CsA)建立同种异体移植免疫抑制组;均为雄性大鼠。RT-PCR半定量检测移植肾AQP2mRNA在各组中表达的变化。结果同种异体移植急性排斥组移植肾AQP2mRNA表达下调,与正常对照组比较有统计学差异(P<0.05),与同系基因对照组和同种异体移植免疫抑制组比较有统计学差异(P<0.05)。同系基因对照组和同种异体移植免疫抑制组中移植肾AQP2mRNA表达的变化与正常对照组比较无统计学差异(P>0.05)。结论AQP2mRNA的表达在急性排斥时表达下调,可以减少对水的重吸收,同时可以减少集合系统能量消耗,有利于移植肾功能的恢复。

Objective To study the change of aquaprorin-2 （AQP2） mRNA in the graft of the rat renal transplantation model, especially after the acute rejection. Methods Normal Wistar rats＇renals were selected as control group, Wistar rats acting as both donors and receptors were selected as syngeneic control group, and SD rats acting as donors but Wistar rats acting as receptors were selected as allogeneic acute rejection group [ given cyclosporine A （CsA） ] and allogeneic immunosuppression group （without given CsA）. All rats were male. RT- PCR method was applied to test the change of AQP2 mRNA expression of each group. Results The AQP2 mRNA expression in allogeneic acute rejection group was decreased significantly compared with that of other three groups （P 〈 0. 05）. Compared with control group, the AQP2 mRNA expression in both syngeneic control group and allogeneic immunosuppression group had no significant change （P 〉 0.05 ）. Conclusions Downregulation of AQP2 mRNA may reduce reabsorption of water and energy consumption in collecting duct of renal transplant in acute rejection phase, which is beneficial to the recovery of renal transplant function.