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RNA干扰Twist基因对人膀胱癌T24细胞化疗敏感性的影响 被引量:2

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摘要 目的构建转录因子Twist基因的短发卡状RNA(shRNA)质粒,转染人膀胱癌T24细胞株后检测T24细胞对羟基喜树碱(HCPT)的敏感性。方法体外构建Twist基因shRNA的表达质粒,脂质体法介导将其转染入T24细胞。实验分为正常对照组、非特异性转染组和特异性转染组。采用RT-PCR法和Western印迹法检测转染后Twist mRNA及蛋白的表达。使用四甲基偶氮唑蓝法(MTT)及流式细胞术(FCM)测定Twist shRNA转染T24细胞后细胞对HCPT敏感性的改变。结果Twist shRNA转染组细胞Twist mRNA和蛋白的表达与其他两组相比明显下调(P<0.05);HCPT敏感性与正常对照组相比明显提高(P<0.05)。结论靶向Twist基因的序列特异性shRNA可增强T24细胞对HCPT的敏感性。
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第8期952-953,共2页 Chinese Journal of Gerontology
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参考文献5

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