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小鼠PARP1基因RNAi表达载体对Lewis细胞株的影响 被引量：1

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摘要 目的观察PARP-1基因RNAi表达载体对Lewis肺癌细胞的抑制及凋亡情况。方法以脂质体Lipofectimine?2000介导,将PARP-1-1308的siRNA表达载体转染Lewis肺癌细胞株,将其分为空白对照组、错义序列组、siRNA组,2Gy照射组、2Gy照射+错义序列组、2Gy照射+siR-NA组。应用MTT法检测转染细胞抑制率、流式细胞术分析转染siRNA后细胞的凋亡,并应用RT-PCR检测转染细胞Parp1mRNA表达水平。结果siRNA转染组及2Gy照射组的吸光度值与空白对照组比较差异有显著意义;2Gy照射组+siRNA组的吸光度值与2Gy照射组、siRNA组比较差异有显著意义。错义序列组与正常对照组比较无显著差异;2Gy照射组+错义序列组与2Gy照射组比较无显著差异。2Gy照射组+siRNA组对Lewis肺癌细胞的抑制率最高。RT-PCR检测结果显示转染细胞Parp1mRNA表达水平降低。siRNA转染、2Gy照射可诱导Lewis肺癌细胞凋亡,与正常对照组比较差异显著(P<0.05),并且siRNA转染可增加2Gy照射引起的Lewis肺癌细胞凋亡。结论针对PARP-1的RNAi可以提高Lewis肺癌细胞的抑制率及放疗引起的肿瘤细胞的凋亡。
作者 王铁君 邹永巍 刘林林 杨海山
机构地区 吉林大学第二医院放疗科 吉林大学口腔医院口腔外科 吉林大学中日联谊医院放疗科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第8期969-970,共2页 Chinese Journal of Gerontology
关键词 PARP RNAI 细胞凋亡 肿瘤细胞
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献11

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二级参考文献12

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同被引文献17

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中国老年学杂志
2009年 第8期

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