反复呼吸道感染患儿血清总IgE和特异性IgE水平

Total Serum IgE and Specific IgE in Children with Recurrent Respiratory Tract Infection

目的:测定反复呼吸道感染(RRTI)患儿血清总IgE(tIgE)水平、特异性IgE(sIgE)阳性率,探讨RRTI与血清tIgE、sIgE的关系。方法:RRTI患儿40例,对照组健康儿童35例,采用酶联免疫吸附(ELISA)法检测血清tIgE水平,欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。结果:(1)RRTI组血清tIgE水平比对照组显著升高(P<0.01);(2)RRTI组血清吸入与食物抗原sIgE类抗体阳性率比对照组明显升高(P<0.01);(3)RRTI组血清sIgE类抗体以抗尘螨组合、猫毛、屋尘抗体多见,而食物组sIgE类抗体则以抗海鱼和淡水鱼抗体多见;(4)sIgE阳性率在tIgE水平升高与否的两组间差异不具显著性,血清tIgE水平升高率在sIgE阳性与否的两组间差异亦不具显著性(P>0.05)。结论:RRTI可能与过敏体质有关,tIgE水平的升高或降低与sIgE阳性率高低并不完全一致。

Objective： To observe the levels of total IgE（tIgE）,and positive rates of specific IgE（sIgE） in diseased children with recurrent respiratory tract infection（RRTI）,and study the relationship of RRTI with tIgE and sIgE.Methods： Blood samples were taken from 40 RRTI children（26 males and 14 females,mean age of 3.68±1.47years） and 35 normal children.Enzyme linked immunosorbent assay（ELISA） method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.Types of sIgE were tested with different allergic antigens.Result：（1）The difference of tIgE levels between RRTI group and control was significant（P〈0.01）;（2） Positive percentage of serum sIgE of RRTI group was significantly higher than that of control group（P 〈0.01）;（3）In serum of RRTI group,the most sIgEs were those against dust acarid-cat hair,and house dust,and the most sIgEs to food antigens were those against sea fish/fresh water fish;（4）There was no correlation between the level of tIgE and positive percentage of sIgE in RRTI group（P〉0.05）.Conclusions：（1）RRTI might correlate with the allergic constitution of the diseased children;（2）It＇s not necessary that the fluctuation of tIgE level coincide with that of sIgE positive rates.