免疫磁珠分离法分选提纯骨髓造血干细胞CD117

Isolation and Purification of Murine Hematopoietic Stem Cell CD117 with Magnetic Activated Cell Sorting Method

目的:探讨用免疫磁珠分离法(magnetic activated cell sorting,MACS)从小鼠股骨、胫骨分离纯化骨髓造血干细胞CD117的方法。方法:收集小鼠骨髓细胞,台盼蓝染色观察其活力,用免疫磁珠分离法分选CD117细胞,流式细胞仪检测荧光标记CD117表达阳性率。结果;未分选前CD117细胞纯度为(41.56±18.77)%,MACS分选CD117细胞纯度(88.68±4.74)%,纯化前后有明显差异(P<0.05)。结论:MACS阳性选择法分选系统能有效分选骨髓造血干细胞CD117。

Objective： To develop a method for purification of CD117 cells from murine bone marrow using magnetic activated cell sorting（MACS） method with immunomagnetic beads.Methods： Bone marrow cells were obtained from mice thighbone and tibia.CD117＋ cells were isolated from these cells using immunomagnetic beads on MiniMACS device,and hematopoietic stem cells（HSCs）were purified.Viability of the collected cells was determined with flow cytometry and light microscopy,respectively.Results： The purity quotient of CD117 HSCs increased from（41.56±18.77）% to（88.68±4.74）% through MACS,and the difference was significant（P〈0.05）.Conclusion： Highly purified CD117 cells can be obtained from murine bone marrow by positive selection using MACS withont any inpairment to the viability of the derived cells.