白桦成熟合子胚快速不定芽诱导体系的建立

Establishment of Rapid Adventitious Bud Induction from Mature Zygotic Embryos of Birch(Betula platyphylla Suk.)

以白桦成熟合子胚为外植体,建立了白桦合子胚快速不定芽诱导体系:合子胚经消毒纵切后,在培养基WPM+2.0mg·L-16-BA+0.2mg·L-1NAA中,可直接诱导形成不定芽,诱导率最高为93.3%,不定芽诱导数为8.7个;WPM+4.0mg·L-1 TDZ上也有较好的诱导效果,不定芽诱导率为90.2%,不定芽诱导数为7.6个,这表明WPM+2.0mg·L-16-BA+0.2mg·L-1 NAA是白桦成熟合子胚不定芽诱导的较优培养基。NAA能有效促进嫩茎生根,其最佳浓度为0.2mg·L-1。

Rapid adventitious bud induction of Betula platyphylla was establinshed from mature zygotic embryos. After disinfect, the zygotic embryos were cut vertically, and induced adventitious buds directly. In the media of WPM＋2.0 mg.L-1 6-BA＋0.2 mg.L-1 NAA, the rate of adventitious buds induction was 93.3%, and number of adventitious buds was 8.7. In the media of WPM＋4.0 mg.L--1 TDZ, the rate was 90.2%, and the number was 7.6. These results indicated that WPM-12.0 mg-L-1 6-BA＋0.2 mg.L-1 NAA was better for induction of adventitious buds. And NAA could promote the rooting of adventitious shoots, the optimum concentration was 0.2 mg.L-1.