茶多酚对实验性动脉粥样硬化兔的血脂及炎症因子的影响

Effects of tea polyphenols on the serum lipid and inflammation factor in experimental atherosclerosis rabbits

目的:探讨茶多酚(TP)对实验性动脉粥样硬化兔的血脂、抗氧化系统及炎症因子的影响。方法:通过喂养高脂饲料建立兔的动脉粥样硬化模型,同时连续i.g.给予茶多酚(100mg·kg-1·d-1)或空白溶媒12周,测定兔血浆中TC、TG、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、NO、C反应蛋白(CRP)、IL-6、TNF-α含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,并观察主动脉病理学形态改变。结果:给予茶多酚可有效减小高脂饲料引起的兔动脉粥样硬化斑块面积,同时降低高脂血症兔血浆TG、TC、LDL-C含量(P<0.01),增加HDL-C含量(P<0.01)及SOD和GSH-Px活力,而且可引起NO、CRP、IL-6、TNF-α含量下降(P<0.01)。结论:茶多酚可以通过降低动脉粥样硬化兔血脂水平、防止脂质过氧化及抑制炎性因子的产生,从而起到抗动脉粥样硬化的作用。

AIM： To investigate the effects of tea polyphenols （TP） on the serum lipid, the anti-oxidant system and inflammaory factor in experimental atherosclerosis （AS） rabbits. METHODS： Experimental AS model of rabbits was made by feeding high fat diet （HFD）. All rabbits were treated with TP （i.g., 100 mg·kg-1·d-1） or vehicle for 12 weeks. The contents ofTC, TG, HDL-C, LDL-C, NO, CRP, IL-6, TNF-α, SOD and GSH-Px in serum were measured. And the pathomorphological changes of aorta were observed. RESULTS ： TP could effectively reduce the area of ath- erosclerotic plaques in HFD treated rabbits. It also de- creased the plasma levels of TC, TG and LDL-C in byperlipemia rabbits（P 〈 0.01）. At the same time, TP treatment lead to the up-regulation of plasma HDL-C levels（P 〈 0.01） and SOD, GSH-Px activity. Moreover, it could decrease the plasma levels of NO, CRP, IL-6 and TNF-a in HFD treated rabbits （ P 〈 0.01 ）. CONCLUSION： The anti-atherogenic effect of TP seems to be related to reducing serum lipids, preventing lipid hyperoxidation and inhibiting the production of imqammatory factor.