Exendin-4对正常动物糖耐量及血糖的影响

Effect of Exendin-4 on the glucose tolerance and serum glucose level in normal animals

目的:研究Exendin-4对健康动物血糖的影响。方法:Wistar大鼠皮下注射Exendin-4(0.1、0.3、0.9μg/kg),每天给药1次,连续给药4周,分别于首次给药后0、1、2、3、4h及2、4周取血,测定大鼠空腹血糖浓度;ICR小鼠皮下注射Exendin-4(0.2、0.6、1.8μg/kg),15min后灌胃给予2.5g/kg葡萄糖,负荷0.5、1、2、3h后取血,测定血糖浓度;小鼠皮下注射Exendin-4(0.2、0.6、1.8μg/kg),15min后,部分灌胃给予2.5g/kg葡萄糖,5min后取血,测定胰岛素浓度。结果:在给药后不同时间内,Exendin-4组大鼠血糖浓度均未见显著变化。在葡萄糖灌胃后,Exendin-4各给药组小鼠血糖浓度跟对照组比较显著降低。Exendin-4给药组小鼠在葡萄糖负荷后,胰岛素水平有显著升高;而无葡萄糖负荷时,胰岛素水平无统计学差异。结论:Exendin-4刺激胰岛素分泌调节血糖的作用是葡萄糖依赖性的。

AIM： To study the effect of Exendin-4 on the glucose tolerance and serum glucose level in normal animals. METHODS： The fasting blood glu- cose concentration was tested at 0,1,2,3,4 h and 2,4 week after the first administration of Exendin-4 （0.1, 0.3, 0.9 g/kg, 4 weeks, qd） in Wistar rats , taking insulin as positive control. Before intragastric adminis- tration 2.5 g/kg glucose, Exendin-4 （0.2, 0.6, 1.8 g/kg） were subcutaneously injected, then the fasting blood glucose concentration was tested at 0.5, 1, 2 h after the glucose loading. After hypodermic administration of Exendin-4 （0.2, 0.6, 1.8 g/kg）, half of the mice were subcutaneously administrated 2.5 g/kg glu- cose 15min later, and the insulin was tested at the end of the experiment. RESULTS： Exendin-4 could not significantly change the fasting blood glucose concen- tration at different times. The fasting blood glucose concentration was significantly decreased after glucose loading by administration Exendin-4. Exendin-4 could increase the serum insulin concentration remarkably after glucose loading and could not change much without glucose loading. CONCLUSION： The results suggest that the blood glucose regulation of Exendin-4 was re- lated to the concentration of glucose.