大熊猫线粒体DNA酶切片段长度多态性研究

Mitochondrial DNA Restriction Fragment Length Polymorphism of Giant Panda

用１２种限制性内切酶（ＢａｍＨⅠ，ＥｃｏＲⅠ，ＥｃｏＲⅤ，ＸｈｏⅠ，ＰｓｔⅠ，ＭｓｐⅠ，ＸｂａⅠ，ＰｖｕⅡ，ＨｉｎｄⅢ，ＨａｅⅢ，ＳａｃⅠ，ＨｐａⅡ）对秦岭大熊猫线粒体ＤＮＡ进行了酶切。测定和分析了每种酶所产生片段的数量和大小，并与四川大熊猫的限制性片段进行了比较，发现在相同的６种酶的１６个酶切位点中有一个不同，表明这两地群体间存在着线粒体ＤＮＡ的多态性。其研究结果为进一步研究和保护大熊猫提供了重要依据。

The mitochondrial DNA of Qinling giant Panda was cleaved with twenty restriction endonucleases: BamH Ⅰ, EcoR Ⅰ, EcoR Ⅴ, Sac Ⅰ, Xho Ⅰ, Pst Ⅰ, Msp Ⅰ, Xba Ⅰ, Pvu Ⅱ, Hind Ⅲ, Hae Ⅲ, Hpa Ⅱ. The number and sizes of cleaved fragments were determined and analyzed for each enzyme. The restriction pattern obtained from Qinling Panda was compared with that from Sichuan. It was found that there was one site difference among 16 restriction sites of six same enzymes, indicating that there was genetic polymorphism between the two local Panda′s populations. The results provided basic imformation and important data for further research and genetic protective management of giant Panda.