福建地区2型糖尿病患者脂联素基因启动子区多态性与颈动脉内膜中层厚度有关

Association of gene polymorphism in promoter region of adiponectin gene and carotid artery intima-media thickness in patients with type 2 diabetes meilitus of Fujian

目的探讨脂联素基因启动子区-11377位点单核苷酸多态性与2型糖尿病患者颈动脉内膜中层厚度（CIMT）之间的关系。方法采用PCR-限制性片段长度多态性（RFLP）技术在504例2型糖尿病患者（CIMT正常组254例，CIMT增厚组250例）中检测脂联素基因-11377位点多态性，同时检测血脂、空腹血糖、空腹胰岛素及血清脂联素水平。结果脂联素基因-11377C→G基因型和等位基因频率在CIMT正常组与CIMT增厚组的分布有显著性差别（P〈0．01）；-11377CC基因型组CIMT值、低密度脂蛋白胆固醇、舒张压、稳态模型评估的胰岛素抵抗指数（HOMA—IR）低于CG＋GG组（P〈0．05或P〈0．01），而血清脂联素水平高于CG＋GG组[（5．70±3．58vs4．72±2．34）mg／L，P〈0．01]。脂联素基因启动子-11377C→G变异与2型糖尿病患者CIMT相关，且独立于脂联素水平（P=0．037）。结论福建地区2型糖尿病患者脂联素基因-11377C→G多态性与CIMT相关。

Objective To explore the association of -11377 site single nucleotide polymorphism in promoter region of adiponectin gene and carotid intima-media thickness （CIMT） in patients with type 2 diabetes mellitus. Methods The adiponectin gene -11377C→G polymorphism was identified by PCR-restriction fragment length polymorphism （RFLP） in 504 patients with type 2 diabetes mellitus （250 patients with increased CIMT and 254 patients with normal CIMT）. Serum lipid and fasting plasma glucose were detected by full automatic biochemical analysor, fasting serum insulin （FINS） was measured by radioimmunoassay, and serum adiponectin level was assessed by ELISA. Results The frequencies of adiponectin gene -11377C→G genotype and allele were different between type 2 diabetic patients with normal and increased CIMT（ P〈0.01 ）. In type 2 diabetic patients, the values of CIMT,diastolic blood pressure, low-density lipoprotein cholesterol （LDL-C） and homeostasis model assessment of insulin resistance （HOMA-IR） in - 11377 CC group were significantly lower than those in - 11377 CG ＋GG group （P〈0.05 or P〈0.01 ）, but serum adiponectin level was higher in -11377CC group than that in -11377CG＋GG group [ （5.70±3.58 vs 4.72±2.34） mg/L, P〈0.01 ]. Variation at adiponectin gene -11377C→ G was associated with increased CIMT in type 2 diabetic patients independent of serum adiponectin level （ P = 0.037）. Conclusion In type 2 diabetic patients of Fujian, the -11377C→G polymorphism of the adiponeetin gene is associated with CIMT.