人腺病毒3型感染对H9c2细胞microRNA表达谱的影响

microRNA expression profile of H9c2 cells infected with human adenovirus type 3

目的研究腺病毒感染对大鼠心肌来源的H9c2细胞microRNA表达谱的影响。方法用人腺病毒3型（Ad3）攻击成层和未成层的H9c2细胞，6h后收获总RNA，基因芯片检测microRNA表达谱。结果比较未成层与成层的H9c2细胞microRNA的表达谱，H9c2细胞成层后有54种microRNA表达显著下调，7种microRNA表达显著上调。与未感染病毒的细胞比较，Ad3感染的未成层H9c2细胞有41种microRNA表达显著下调，12种microRNA表达显著上调。与未感染病毒的细胞比较，Ad3感染成层H9c2细胞后有8种microRNA的表达显著下调，62种microRNA的表达显著上调。结论细胞不同的生长状态及Ad3感染显著改变了H9c2细胞的microRNA表达谱。

Objective To study the microRNA expression profile of rat myocardium-derived H9c2 cells infected with human adenovirus type 3 （ Ad3 ）. Methods Confluent and unconfluent H9c2 cells were attacked with Ad3. Six hour later total RNAs were harvested and microRNA expression profiling was analyzed by microRNA array. Results The expression of 54 microRNAs was down-regulated and 7 microRNAs was up-regulated in confluent H9c2 cells compared with that in unconfluent H9c2 cells. The expression of 41 microRNAs was down-regulated and 12 was up-regulated in unconfluent H9c2 cells infected with Ad3 compared with that in unconfluent H9c2 cells without Ad3 infection. There were 8 microRNAs down-regulated and 62 upregulated in confluent H9c2 infected with Ad3 compared with that in confluent H9c2 cells without Ad3 infection. Conclusion The microRNA expression profile, correlating with different cell growth states, was significantly altered by human adenovirus infection.