鳙鱼不同组织的同工酶研究

STUDY IN THE ISOZYMES OF DIFFERENT TISSUES IN BIGHEAD CARP(ARISTICHYS NOBILIS)

采用聚丙烯酰胺凝胶电泳的方法，对鳙鱼的脑、晶体、心脏、肝脏、肾脏、肌肉等六种组织进行了十种同工酶（α＿ＡＭＹ、ＥＳＴ、ＧＯＴ、Ｇ６ＰＤ、ＩＤＨ、ＬＤＨ、ＭＤＨ、ＭＥ、ＰＯＸ、ＳＯＤ）的电泳研究，并对各种酶的同工酶位点及酶谱表型进行了分析，其中鳙鱼的α＿ＡＭＹ和ＰＯＸ的研究还未见报道．结果表明，除ＧＯＴ外，其余酶均存在不同程度的组织差异，Ｇ６ＰＤ在肝脏，ＩＤＨ在脑以及ＬＤＨ在心脏出现了特殊的酶谱，作者就此进行了分析和推测．

By using the vertical polyacrylamide gel electrophoresis, ten isozymes (α_AMY, EST, GOT, G6PD, IDH, LDH, MDH, ME, POX, SOD) of bighead carp (Aristichys nobilis) from six differentiated adult tissues (brain, eye, heart, kidney, liver, muscle) are studied and the loci and phenotypes of the ten isozymes are analysed. Among the enzymes studied, α_AMY and POX have not found being reported in the bighead carp. The results show that all isozymes, except GOT, present a specific tissue distribution. There are some specific patterns in the G6PD (liver), IDH (brain) and LDH (heart) and the genetic principle of these phenomenon are discussed and conjectured.